2D diagonal redox SDS-PAGE of Paleo Supplements proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).Both dimensions consist of a conventional SDS-PAGE, except Colloidal Minerals that the sample buffer for the first dimension lacks a reducing agent.
Intermolecular disulfide bridges increase the apparent molecular weight of a protein in the first dimension, whereas intramolecular bridges decrease the apparent weight of the protein.